Skip to main content
. 2022 Nov 4;56(6):e13355. doi: 10.1111/cpr.13355

FIGURE 1.

FIGURE 1

Culture system establishment of human gastric epithelial progenitor‐like (hGEPL) cells. (A) Primary human epithelial cells cultured in different media on Day 6. Scale bar: 100 μm. (B) Primary human epithelial cells cultured in 2D, 3D and GM modes. Scale bar: 100 μm. (C) Cell proliferation analysis for primary human epithelial cells cultured in 2D, 3D and GM modes after serial culture. (D) The expression levels of representative genes in primary human epithelial cells cultured in 2D, 3D and GM modes after serial culture. (E) Immunofluorescence staining of FABP5, MUC5AC and MKI67 on hGEPL cell line. Scale bar: 20 μm. (F) hGEPL cell line cultured in different culture media (Chem, Chem+C, Chem+V and Chem+CV). Scale bar: 100 μm. (G) The expression levels of LGR5 and RBPJ in hGEPL cell line cultured with Chem+C and Chem+V, respectively. Significance was determined by t‐tests. *p value < 0.05. (H) Cycling score of hGEPL cell line cultured in different culture media (Chem, Chem+C, Chem+V and Chem+CV). (I) hGEPL cells after serial passages. Scale bar: 100 μm. (J) The expression levels of representative genes in hGEPL cells after serial passages.