Skip to main content
. 2023 May 17;26(1):328. doi: 10.3892/etm.2023.12027

Figure 5.

Figure 5

miR-140-3p is negatively correlated with the expression of TRAF2. (A) RT-qPCR analysis verifying the effectiveness of 3 siRNAs against TRAF2 in MCF-7 and MDA-MB-231 cells. *P<0.05 vs. si-NC. (B) Western blot analysis verifying the effectiveness of 3 siRNAs against TRAF2 in MCF-7 and MDA-MB-231 cells. *, si-TRAF2-1, si-TRAF2-2,si-TRAF2-3 groups vs. si-NC group. (C) RT-qPCR analysis of the effect of SCO combined with miR-140-3p inhibitor on the expression of TRAF2. *P<0.05. (D) RT-qPCR analysis of the effect of si-TRAF2 on miR-140-3p expression. (E) Western blot analysis of the effect of si-TRAF2 on protein levels of TRAF2 in the presence of miR-140-3p inhibitor. *P<0.05 vs. inhibitor + si-NC. SCO, scoparone; TRAF2, receptor-associated factor 2; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; miR, microRNA; Bio, biotinylated; ceRNA, competing endogenous RNA; si, small interfering.