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. Author manuscript; available in PMC: 2023 Jun 20.
Published in final edited form as: ACS Chem Biol. 2022 Nov 3;17(11):3229–3237. doi: 10.1021/acschembio.2c00706

Figure 1 ∣. Lenalidomide sequesters eIF3i from the eIF3 complex.

Figure 1 ∣

(a) Overview of the structural and mechanistic foci in this study. Coimmunoprecipitation of (b) endogenous CRBN, (c) endogenous eIF3i, and (d) endogenous eIF3b from HUVECs. (e) Representative sucrose gradient analysis of purified eIF3i-Flag complexes with or without lenalidomide treatment. Samples were separated to 11 fractions, and the eIF3i-eIF3b and CRBN–lenalidomide–eIF3i complex were examined by immunoblotting.