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. 2000 Apr 1;28(7):e23. doi: 10.1093/nar/28.7.e23

Figure 2.

Figure 2

Screening of BAC-RF blots for two DNA probes. Sorghum genomic DNA probes SHO74 (a) and pSB1172 (b) were applied to S.bicolor (genotype BTx623; lane 1), S.propinquum (unnamed accession; lane 2), and 14 BAC pools each comprised of 384 clones, collectively representing about one-genome coverage of S.propinquum. Previous RFLP mapping of SHO74 showed that the 9.4 kb S.bicolor band was allelic to the 4.3 kb S.propinquum band, and that the locus maps to linkage group D. While the additional bands could be faintly discerned, they could not be mapped. Pools 2 and 13 (lanes 4 and 15) clearly contain BACs that harbor the additional loci, while pools 6 and 12 (lanes 8 and 14) each contain BACs that correspond to the mapped locus. pSB1172 shows a smear in the parental genotypes (even with shorter exposures), which is dissected into individual constituent loci in the BAC pools.