Table 1. DNA fingerprint analysis of locus-specific groups of BACs as determined by the BAC-RF method. (A) Sorghum BACs. (B) Sugarcane BACs.
| A. Sorghum BACs | ||||||
|---|---|---|---|---|---|---|
| |
|
|
|
|
|
|
| Locus | Clone | Best internal match | Best cross-match | |||
| Clone | Score (a) | Clone | Score (b) | Log (a/b) | ||
| pSB1140a | 10A14 | 42F20 | 2e-24 | 04A14 | 2e-08 | –16.0 |
| pSB1140a | 42F20 | 10A14 | 2e-24 | 04A14 | 6e-11 | –13.5 |
| pSB1140a | 42L10 | 42F20 | 2e-21 | 04A14 | 1e-10 | –10.7 |
| |
|
|
|
|
Average |
–13.4 |
| pSB1140b | 34N18 | 64A06 | 4e-14 | 32J21 | 7e-13 | –1.2 |
| pSB1140b | 55E08 | 64A06 | 5e-20 | 98E20 | 2e-07 | –12.6 |
| pSB1140b | 64A06 | 55E08 | 5e-20 | 32J21 | 3e-11 | –8.8 |
| Average | –7.5 | |||||
| pSB1140c | 62L04 | 83N10 | 1e-22 | 56H18 | 2e-11 | –11.3 |
| pSB1140c | 83N10 | 92A13 | 8e-24 | 41E03 | 6e-09 | –14.9 |
| pSB1140c | 92A13 | 83N10 | 8e-24 | 10K22 | 2e-10 | –13.4 |
| |
|
|
|
|
Average |
–13.2 |
| pSB1698a | 10K22 | 32K17 | 1e-15 | 92A13 | 2e-10 | –5.3 |
| pSB1698a | 32J21 | 32K17 | 5e-19 | 34N18 | 7e-13 | –6.1 |
| pSB1698a | 32K17 | 32J21 | 5e-19 | 12O13 | 7e-10 | –9.1 |
| pSB1698a | 41E03 | 62G07 | 8e-28 | 04A14 | 7e-10 | –17.9 |
| pSB1698a | 41G18 | 41E03 | 1e-22 | 34N18 | 5e-11 | –11.7 |
| pSB1698a | 62G07 | 41E03 | 8e-28 | 04A14 | 2e-11 | –16.4 |
| pSB1698a | 74O08 | 85O16 | 1e-25 | 70I16 | 8e-09 | –16.9 |
| pSB1698a | 85O16 | 74O08 | 1e-25 | 42L10 | 2e-07 | –18.3 |
| pSB1698a | 89J06 | 85O16 | 3e-18 | 62L04 | 2e-08 | –9.8 |
| |
|
|
|
|
Average |
–12.4 |
| pSB1698c | 04A14 | 56H18 | 1e-32 | 62G07 | 2e-11 | –21.3 |
| pSB1698c | 56H18 | 04A14 | 1e-32 | 62L04 | 2e-11 | –21.3 |
| pSB1698c | 70I16 | 56H18 | 2e-17 | 41G18 | 4e-10 | –7.3 |
| pSB1698c | 92O11 | 04A14 | 3e-30 | 42L10 | 2e-10 | –19.8 |
| Average | –17.4 | |||||
| pSB1698d | 12O13 | 98E20 | 6e-25 | 04A14 | 6e-10 | –15.0 |
| pSB1698d | 48D01 | 98E20 | 1e-16 | 04A14 | 2e-09 | –7.3 |
| pSB1698d | 60B15 | 98E20 | 9e-24 | 32K17 | 3e-08 | –15.5 |
| pSB1698d | 98E20 | 12O13 | 6e-25 | 04A14 | 4e-09 | –15.8 |
| |
|
|
|
|
Average |
–13.4 |
| pSB1698e | 67E04 | 96D19 | 4e-10 | 92O11 | 3e-10 | 0.1 |
| pSB1698e | 96D19 | 67E04 | 4e-10 | 70I16 | 5e-10 | –0.1 |
| |
|
|
|
|
Average |
0.0 |
| Per group average Log (a/b) | –11.1 | |||||
| Per BAC average Log (a/b) | –12.0 | |||||
| Average no. BACs per locus | 3.1 | |||||
| B. Sugarcane BACs | ||||||
|---|---|---|---|---|---|---|
| |
|
|
|
|
|
|
| Locus | Clone | Best internal match | Best cross-match | |||
| Clone | Score (a) | Clone | Score (b) | Log (a/b) | ||
| SH2a | 13A18 | 161B11 | 7E-05 | 87I1 | 2E-06 | 1.5 |
| SH2a | 161B11 | 199A10 | 2E-09 | 38M8 | 1E-06 | –2.7 |
| SH2a | 199A10 | 238N13 | 6E-13 | 130F11 | 7E-07 | –6.1 |
| SH2a | 238M13 | 199A10 | 6E-13 | 12A1 | 9E-06 | –7.2 |
| |
|
|
|
|
Average |
–3.6 |
| SH2b | 12A1 | 130F11 | 8E-12 | 9B1 | 5E-13 | 1.2 |
| SH2b | 54I3 | 130F11 | 1E-17 | 9B1 | 5E-08 | –9.7 |
| SH2b | 130F11 | 54I3 | 1E-17 | 9B1 | 3E-08 | –9.5 |
| |
|
|
|
|
Average |
–6.0 |
| SH2c | 133J7 | 235B15 | 3E-17 | 87I1 | 6E-07 | –10.3 |
| SH2c | 174G16 | 133J7 | 2E-07 | 87I1,166O17 | 1E-04 | –2.7 |
| SH2c | 235B15 | 133J7 | 3E-17 | 51N2 | 3E-06 | –11.0 |
| |
|
|
|
|
Average |
–8.0 |
| SH2d | 54N6 | 52I37 | 7E-07 | N.A. | ||
| SH2e | 3M6 | 182N4 | 4E-06 | 235B15 | 1E-04 | –1.4 |
| SH2e | 7B15 | 91D11 | 2E-07 | 179N14 | 2E-05 | –2.0 |
| SH2e | 7B16 | 7B15 | 4E-05 | 38K14,235B15 | 2E-04 | –0.7 |
| SH2e | 51N2 | 52I3 | 1E-19 | 143N19 | 6E-14 | –5.8 |
| SH2e | 52I3 | 51N2 | 1E-19 | 143N19 | 6E-14 | –5.8 |
| SH2e | 91D11 | 7B15 | 2E-07 | 38K14 | 2E-06 | –1.0 |
| SH2e | 143N19 | 177O18 | 2E-19 | 63G10 | 2E-06 | –13.0 |
| SH2e | 146B3 | 52I3 | 4E-06 | 26K6,54N6 | 2E-06 | 0.3 |
| SH2e | 152G10 | 51N2,52I3 | 5E-04 | 68H8 | 3E-05 | 1.2 |
| SH2e | 160O6 | 177O18 | 8E-08 | 87I1 | 6E-07 | –0.9 |
| SH2e | 143N19 | 182N4 | 1E-21 | 143N19 | 2E-19 | –2.3 |
| SH2e | 182N4 | 177O18 | 1E-21 | 143N19 | 1E-14 | –7.0 |
| SH2e | 198L13 | 216J5 | 6E-12 | 133J17 | 8E-06 | –6.1 |
| SH2e | 216J5 | 198L13 | 6E-12 | 94E5′ | 6E-06 | –6.0 |
| SH2e | 230F15 | 240M3 | 6E-10 | 63G10 | 6E-07 | –3.0 |
| SH2e | 240M3 | 230F15 | 6E-10 | 63G10 | 2E-07 | –2.5 |
| |
|
|
|
|
Average |
–3.5 |
| SH2f | 22D5 | 22M20 | 4E-23 | 54I3 | 6E-05 | –18.2 |
| SH2f | 22M20 | 22D5 | 4E-23 | 54I3 | 6E-06 | –17.2 |
| SH2f | 26K6 | 65G1 | 3E-02 | 146B3 | 2E-06 | 4.2 |
| SH2f | 29G4 | 148B7 | 6E-14 | 7B15,54I3,4O5 | 1E-03 | –10.2 |
| SH2f | 38I8 | 22D5 | 2E-11 | 161B11 | 5E-06 | –5.4 |
| SH2f | 38K14 | 38I8 | 5E-08 | 91D11 | 2E-06 | –1.6 |
| SH2f | 38M8 | 38I8 | 2E-08 | 179N14 | 3E-09 | 0.8 |
| SH2f | 63G10 | 38K14 | 7E-04 | 240M3 | 2E-07 | 3.5 |
| SH2f | 65G1 | 148B7 | 4E-05 | 143N19 | 3E-05 | 0.1 |
| SH2f | 94E5′ | 148B7 | 7E-11 | 216J5 | 6E-06 | –4.9 |
| SH2f | 148B7 | 29G4 | 6E-14 | 143N19 | 1E-06 | –7.2 |
| |
|
|
|
|
Average |
–5.1 |
| SH2g | 7C7 | 87I1 | 4E-04 | 5C19,19K12 | 1E-05 | 1.6 |
| SH2g | 66E14′ | 87I1 | 5E-03 | 13A18 | 5E-05 | 2.0 |
| SH2g | 87I1 | 7C7,99D20 | 7E-04 | 133J7,160O6 | 6E-07 | 3.1 |
| SH2g | 99D20 | 87I1 | 7E-04 | 179N14 | 1E-06 | 2.8 |
| |
|
|
|
|
Average |
2.4 |
| SH2h | 9B1 | 240H12 | 4E-03 | 12A1 | 5E-13 | 9.9 |
| SH2h | 212D23 | 240H12 | 1E-05 | 7B16 | 3E-03 | –2.5 |
| SH2h | 240H12 | 212D23 | 1E-05 | 62L4 | 2E-02 | –3.3 |
| |
|
|
|
|
Average |
1.4 |
| CDSR029a | 17P9 | 62L4 | 5E-11 | 68H8 | 2E-10 | –0.6 |
| CDSR029a | 62L4 | 17P9 | 5E-11 | 68H8 | 1E-15 | 4.7 |
| |
|
|
|
|
Average |
2.0 |
| CDSR029b | 5C19 | 5C20 | 2E-23 | 4O5 | 5E-06 | –17.4 |
| CDSR029b | 5C20 | 5C19 | 2E-23 | 146B3 | 3E-06 | –17.2 |
| CDSR029b | 19K12 | 5C20 | 2E-11 | 4O5 | 2E-06 | –5.0 |
| CDSR029b | 161B16 | 5C19 | 7E-09 | 187M21 | 5E-06 | –2.9 |
| |
|
|
|
|
Average |
–10.6 |
| CDSR029c |
166O17 |
|
|
140E14′ |
1E-05 |
N.A. |
| CDSR029d | 4O5 | 440E14′ | 1E-11 | 19K12 | 2E-06 | –5.3 |
| CDSR029d | 107M24 | 440E14′ | 1E-03 | 199A10 | 1E-05 | 2.0 |
| CDSR029d | 140E14′ | 4O5 | 1E-11 | 187M21 | 2E-07 | –4.3 |
| |
|
|
|
|
Average |
–2.5 |
| CDSR029e | 68H8 | 179N14 | 3E-12 | 62L4 | 1E-15 | 3.5 |
| CDSR029e | 179N14 | 68H8 | 3E-17 | 62L4 | 1E-11 | –5.5 |
| |
|
|
|
|
Average |
–1.0 |
| CDSR029f |
187M21 |
|
|
52I3 |
7E-07 |
N.A. |
| Per group average Log a/b | –3.1 | |||||
| Per BAC average Log a/b | –3.7 | |||||
| Average no. BACs per locus | 4.1 | |||||