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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Antiviral Res. 2023 May 5;215:105624. doi: 10.1016/j.antiviral.2023.105624

Fig. 4. Viral specificity of a CRISPR-Cas12a assay.

Fig. 4.

The CMV targets UL123 and US28 were tested for specificity against HSV-1, HHV-6A, HHV-6B and the CMV strains Towne, TR, and Toledo. Virus was spiked in nuclease free water at 105 IU/mL followed by DNA extraction and PCR amplification. Samples were then tested using a CRISPR-Cas12a assay. Specificity using A) UL123 and B) US28 were assayed. N = 3. Positive is calculated by the mean of the NTC + (3*SD) and is indicated by the dotted line. *(P < 0.05), ***(P < 0.001), ****(P < 0.001) statistical significance determined using a one-way ANOVA with a Dunnett’s multiple comparison test single pooled variance. IU = infectious units.