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. Author manuscript; available in PMC: 2023 Jun 20.
Published in final edited form as: Nat Cell Biol. 2023 Jan 16;25(2):309–322. doi: 10.1038/s41556-022-01055-y

Extended Data Fig. 4: LPAR4 upregulates ECM genes and cancer stemness-related genes in the absence of exogenous LPA.

Extended Data Fig. 4:

a, Quantitative RT-PCR confirmation of LPAR4 regulated genes associated with ECM in two additional pairs of +EV vs. +R4 cells, grown in charcoal stripped FBS containing media. All mRNA level was normalized to +EV cells. b, Graphs showing the relative number of viable 79E+EV or 79E+R4 cells treated with various doses of H2O2 or gemcitabine in serum-free media for 72 hours, evaluated by the CellTiter-Glo assay. c, Graph showing the number of tumorspheres formed by Colo-34+EV or Colo-357+R4 cells grown in 3D suspension with serum-free media at day 10. The right panel shows representative images from three biological experiments for spheres formed by +EV or +R4 cells at day 10. d, Relative gene expression of CSC markers and antioxidant genes in +EV vs. +R4 cells grown in charcoal stripped FBS containing media. All mRNA level was normalized to +EV cells. Data were presented as mean ± s.d. for n=3 independent experiments (a-d). Statistical analyses were performed using two tailed unpaired one sample t-test (a, c, and d). Source numerical data are available in source data.