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. Author manuscript; available in PMC: 2023 Jun 20.
Published in final edited form as: Nat Cell Biol. 2023 Jan 16;25(2):309–322. doi: 10.1038/s41556-022-01055-y

Fig. 5: LPAR4 expression promotes the cell-autonomous production of FN1.

Fig. 5:

a, Expression correlation between LPAR4 and FN1 from the TCGA PAAD dataset (n=177 samples). Pearson correlation coefficient r=0.63 and two-tailed P <0.0001. b, Expression correlation between LPAR4 and FN1 from the PDX tumors from the orthotopic mouse models treated with vehicle control (n=3 biologically independent samples) or gemcitabine (n=5 biologically independent samples). Pearson correlation coefficient r=0.80 and two-tailed P=0.018. c, Overall and relapse-free survival for patients with low vs. high FN1 expression in the TCGA PAAD dataset. d, Representative images for human FN1 immunohistochemistry staining in 79E (n=10 biologically independent samples), Colo-357 (n=4 biologically independent samples), or 34E (n=5 biologically independent samples) xenograft tumors formed from 1 million (1M) cells injected or 300 cells injected. Scale bar is 50 μm. e-f, Western blot and immunostaining showing FN1 protein expression for pancreatic cancer cells with stable ectopic expression of LPAR4 (+R4) vs. empty vector control (+EV) grown in charcoal striped FBS containing media for 72 hours. Data are representative of three biological experiments. g, Representative images for human FN1 immunohistochemistry staining in Colo-357+EV vs. Colo-357+R4 tumors harvested at day 15 post implantation in the pancreas of nu/nu mice. Images in the lower panel highlight FN1 staining in matrix areas. Data are representative of three biological samples. Scale bar is 100 μm or 20 μm as indicated. h, Effects of hypoxia and LPAR4 knockdown on FN1 protein expression. Data are representative of three biological experiments. Source numerical data and unprocessed blots are available in source data.