Table 1. Summary of vectors described in this work.

Plasmid name, brief description, bacterial antibiotic resistance, Addgene stock number, and bibliographic reference are indicated for all plasmids mentioned in this work. Addgene, public plasmid repository (https://www.addgene.org/)

Plasmid name	Description	Resistance	Addgene	Reference
pUPD	Universal Part Domesticator plasmid: “Domestication” level plasmid to adapt parts to Goldenbraid 2.0	AmpicillinR	NA	(Sarrion-Perdigones et al., 2011)
pUPD2	Universal Part Domesticator plasmid 2: “Domestication” level plasmid to adapt parts to Goldenbraid 2.0	AmpicillinR	165856	(Matinyan et al., 2021b)
pUPD3	Universal Part Domesticator plasmid: 3”Domestication” level plasmid to adapt parts to Goldenbraid 2.0	ChloramphenicolR	118043	(Sarrion-Perdigones et al., 2019)
pAlpha1	Alpha level destination plasmid 1: “Alpha” level Goldenbraid 2.0 plasmid	KanamycinR	118044	(Sarrion-Perdigones et al., 2019)
pAlpha2	Alpha level destination plasmid 2: “Alpha” level Goldenbraid 2.0 plasmid	KanamycinR	118045	(Sarrion-Perdigones et al., 2019)
pOmega1	Omega level destination plasmid 1: “Omega” level Goldenbraid 2.0 plasmid	ChloramphenicolR	118046	(Sarrion-Perdigones et al., 2019)
pOmega2	Omega level destination plasmid 2: “Omega” level Goldenbraid 2.0 plasmid	ChloramphenicolR	118047	(Sarrion-Perdigones et al., 2019)
pDmHsp70B_TACT	“Domesticated part” plasmid encoding the Drosophila melanogaster Hsp70 promoter	AmpicillinR	165780	(Matinyan et al., 2021b)
pCP6_Dros	“Domesticated part” plasmid encoding the synthetic Escherichia coli CP6 promoter containing a Drosophila melanogaster Kozak/Cavener translational initiation sequence	AmpicillinR	165794	(Matinyan et al., 2021b)
pNPTII	“Domesticated part” plasmid encoding a Drosophilized Neomycin phosphotransferase II of transposon Tn5	AmpicillinR	165836	(Matinyan et al., 2021b)
pHSV-TK	“Domesticated part” plasmid encoding a minimal polyadenylation signal of the thymidine kinase gene from the herpes simplex virus	AmpicillinR	165801	(Matinyan et al., 2021b)
pR70B04	“Domesticated part” plasmid encoding the R70B04 enhancer from Drosophila melanogaster	AmpicillinR	165792	(Matinyan et al., 2021b)
pdSCP	“Domesticated part” plasmid encoding the Drosophila melanogaster synthetic core promoter	AmpicillinR	165776	(Matinyan et al., 2021b)
pLexA BD	“Domesticated part” plasmid encoding the Drosphilized DNA binding domain of the LexA repressor from Escherichia coli	AmpicillinR	165821	(Matinyan et al., 2021b)
pGGGSx4	“Domesticated part” plasmid encoding the Drosphilized peptide linker (GlyGlyGlySer)4	AmpicillinR	165807	(Matinyan et al., 2021b)
pVP16 AD	“Domesticated part” plasmid encoding the Drosphilized transcription factor activation domain of VP16 from the herpes simplex virus	AmpicillinR	165822	(Matinyan et al., 2021b)
pSV40L	“Domesticated part” level plasmid encoding the late polyadenylation signal from simian vacuolating virus 40	AmpicillinR	165798	(Matinyan et al., 2021b)
pFC31 attB O1	“Omega” level plasmid containing the attB site from the bacteriophage phiC31 cloned in Omega level destination plasmid 1	ChloramphenicolR	165843	(Matinyan et al., 2021b)
pMiniWhiteA2	“Alpha” level plasmid encoding the dominant “eye” screening marker called “mini-white” from Drosophila melanogaster cloned in Alpha level destination plasmid 2	KanamycinR	165873	(Matinyan et al., 2021b)