Fig. 4.

Improving biopanning parameters. (a) Quantification of number of yeast cells expressing pBEVY-pT231 scFv 3.24-GFP present in wells when tested across different biotin concentrations. Each datapoint indicates a value measured from a biological replicate in an experiment (3 separate experiments). Error bars indicate standard error of means of the datapoints. (b) Normalized biopanning ‘signal’ [(# yeast cells, phospho-peptide wells)-(# yeast cells, no peptide wells)]/(# yeast cells, highest concentration of peptide) for a range of peptide concentrations. Each datapoint indicates average of values measured from three separate experiments with three biological replicates in each experiment (3 separate experiments). Error bars indicate standard deviation. (c) Number of yeast cells present in wells when tested across different streptavidin concentrations. Each datapoint indicates average of values measured from two biological replicates in an experiment (3 separate experiments). Error bars indicate standard deviation of the datapoints. For panels (a) and (c), ^**P ≤ 0.01, ^***P ≤ 0.001, ^****P ≤ 0.0001 using Tukey’s multiple comparisons test. Otherwise, P > 0.05.