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Biopanning yeast cells against p-tau in 6-well plates. Ratios of the number of yeast cells expressing pBEVY-pT231 scFv Y31A-GFP to yeast cells expressing pBEVY-4420-mCherry were measured using flow cytometry. Results from an initial ratio of Y31A:4420 = 1:1000 (a) and 1:100 000 (b). % GFP positive indicates the percentage of yeast cells expressing GFP among all yeast cells recovered after each round.
