Figure 5:
DLin-MC3-DMA conjugate enhances endosomal escape in cell culture. (A) Fluorescence microscopy of Gal8-YFP reporter cells after 24h incubation of media only, RNAiMAX only, or conjugated siRNAs. (B.) Counted number of large foci Gal8+ and Gal8− cells for each treatment group (n>300 counted per group) (C.) Quantification of average Gal8-YFP fluorescence intensity per cell. Error bars represent SEM (n = >300 cells counted per group). Data analysis: Multiple comparisons = One-way ANOVA, Dunnett test (****P<0.0001, *P<0.1).