DLin-MC3-DMA conjugate does not induce non-specific gene modulation in tissues accumulating less than 20 pmol of siRNA/mg of tissue. Observed Htt and Ppib mRNA silencing (A.) normalized to Hprt (an housekeeping gene) (B.) non normalized to Hprt in heart, fat, and muscle after SC injection of DLin-MC3-DMA siRNAs (1 week, 20 mg/kg, n=5-6, mean ± SD) (C.) Bar graph showing Hprt mRNA silencing in heart, fat, and muscle after SC injection of DLin-MC3-DMA siRNAs (1 week, 20 mg/kg, n=5-6, mean ± SD). Data analysis: Multiple comparisons = One-way ANOVA, Dunnett test (**P<0.01, *P<0.1).