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. Author manuscript; available in PMC: 2023 Jun 20.
Published in final edited form as: Nat Protoc. 2023 Jan 27;18(3):929–989. doi: 10.1038/s41596-022-00754-y

Fig. 14 |. Detection of DNA crosslinks in a theoretical cell culture study.

Fig. 14 |

Experiments are first carried out to find a suitable level of DNA SBs, using an agent that directly causes breaks in DNA such as H2O2 or ionizing radiation (left). Subsequently, experiments are done where cells are exposed to the test agent (compound) and ionizing radiation. The presence of crosslinks in DNA is concluded if the irradiated samples plus the tested compound have less DNA migration as compared with the irradiated samples without the tested compounds (black bars compared with gray bars).