Table 2. Comparison of statistics between automatically gathered (server gathered) and manually gathered statistics for maximum Cα displacement and maximum rotation.

	Hand-gathered statistics			Automatically collected motion statistics
Value	Min.	Max.	Mean	Min.	Max.	Mean	Median	SD
Maximum Cα displacement (Å)	1.5	60	12	0.90	81	23	17	19
Maximum hinge rotation (°)	5	148	24	0.0	150	35	9.5	46

Despite the sparseness of the manually culled data, the statistics are roughly comparable. Maximum Cα displacement was calculated by first sieve-fitting the protein conformations. The 81 Å motion in the database is due to Oxo-Acid-Lyase (5CTS to 1AJ8 in the PDB). The 12 references reporting maximum rotation in the literature reported a mean maximum rotation of 24°, whereas the server found a mean maximum rotation of 35° over the 176 entries present at the time the table was generated. The mean, however, is skewed by some of the larger motions; the median displacement is much smaller. The maximum value of 150° is due to Oxidoreductase (1FMC→1HDC in the PDB). To collect the manual data, we found 11 entries in the Database of Macromolecular Motions citing manually gathered Cα displacement statistics from the literature, and 12 entries giving manually gathered maximum hinge rotations. (Some researchers reported only Cα displacement while others reported only maximum hinge rotation, so these correspond to different sets of proteins.) Automatic collection used a sample of 184 motions for Cα displacement and 176 motions for maximum hinge rotation.