Figure 2.

Ectopic expression of TRIM13 suppressed LUAD cells proliferation and facilitated cell apoptosis. LUAD cells were transfected with TRIM13 and its mutated version.

Note: (a) The mRNA expression of TRIM13 in A549 and HCC827 cell lines was detected using RT-qPCR after transfection with oe-TRIM13 and its mutated version and compared with control cells. (b) The transfection efficacy of oe-TRIM13 and oe-TRIM13-ΔRING was detected in LUAD cells using western blot analysis. (c) Colony formation assay showed reduced growth in LUAD cells transfected with oe-TRIM13 as compared to control and mutated version. (d) CCK-8 assay was performed to evaluate LUAD cell viability and proliferation by measuring OD absorbance. Results showed that oe-TRIM13-A549 and oe-TRIM13-HCC827 cells grew more slowly than oe-NC and oe-TRIM13-ΔRING cells. (e) TRIM13 expression increased apoptosis in LUAD cells. The apoptotic rate of transfected cells was determined using flow cytometry. (f) The protein levels of Bax, Bcl-2, Cyclin A1, and Cyclin D1 in LUAD cells transfected with oe-TRIM13 and oe-TRIM13-ΔRING were detected using western blot analysis. The data represents the mean ± S.D. from three independent experiments, and statistical significance was determined using ANOVA test with ** P < 0.01 and ***P < 0.001.