Figure 6.

TRIM13 regulates the Keap1/Nrf2 pathway in LUAD cells.

Note: (a) Western blot analysis was performed to determine the protein levels of Keap1/Nrf2 pathway-related proteins in LUAD cells transfected with oe-NC or oe-TRIM13. Results showed that TRIM13 overexpression increased Keap1 protein levels while reducing both cytoplasmic and nuclear Nrf2 expression, indicating inactivation of the Nrf2 pathway. (b) RT-qPCR analysis demonstrated that the mRNA levels of downstream antioxidant enzymes of Nrf2, such as HO-1 and NQO1, were significantly lower in oe-TRIM13-transfected LUAD cells compared to control cells. The statistical significance was set at * P < 0.05, ** P < 0.01, and ***P < 0.001.