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. 2023 May 28;22(12):1496–1513. doi: 10.1080/15384101.2023.2216504

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Figure 7. — TRIM13 regulated LUAD cell malignancy through NRF2.

Note: (a) The transfection efficacy of oe-NRF2 in A549 cells was determined by RT-qPCR. (b) Colony formation assay revealed reduced LUAD cell proliferation in oe-TRIM13 group compared to oe-NC or oe-TRIM13+oe-Nrf2 group. (c) Cell viability was assessed by CCK-8 in all constructs. (d-e) Flow cytometry analysis demonstrated reduced LUAD cell apoptosis following Nrf2 transfection. (f) TEM images were captured, and autophagosomes in LUAD cells (marked by red arrows) were counted in each construct. NRF2 expression resulted in a decrease in the autophagosome level in A549 LUAD cells, partially reversing the effects of TRIM13. (g) LC3 staining was performed to detect LUAD cell autophagy. (h) DCFH-DA staining was used to measure LUAD cell oxidative stress. **P < 0.01, ***P < 0.001 and #P < 0.05, ##P < 0.01, ###P < 0.001 were considered significant.