Figure 8.

TRIM13 suppressed LUAD tumor growth through NRF2 in vivo.

Note: (a) Xenografts injected with TRIM13-overexpressing A549 cells showed a significant reduction in tumor volume. (b) Tumors derived from TRIM13-overexpressing A549 cells exhibited reduced weight in each mouse, whereas the presence of NRF2 increased the tumor weight. The images of tumors in panel B illustrate the reduced tumor size due to TRIM13 overexpression. (c) Immunohistochemical analysis of Ki67 expression in mouse xenograft tissues showed reduced tumor cell proliferation due to TRIM13 overexpression, as revealed by quantitative results presented in the graph (d). In E illustration, TUNEL staining of mouse xenografts and corresponding quantitative data indicated an increase in apoptotic events in the presence of TRIM13, which were reduced by NRF2. (f) IHC of p62 staining in mouse xenografts and corresponding quantitative data revealed a significant reduction in p62-positive cells in the presence of TRIM13, which was reversed by NRF2. Statistical significance was denoted by ***P < 0.001, #P < 0.05, and ###P < 0.001.