Fig. 2.
High-throughput RNAi screen identifies regulators of CENP-A mislocalization. (A) Bar chart showing the ratio between mean YFP signal intensities and mean number of nuclei in HeLaYFP–CENP-A-high cells transfected with the indicated siRNAs for 72 h in 384-well plates. (B) Median Z-score of lead candidate genes obtained from the primary screen of an siRNA chromatin library for increased nuclear YFP intensity. (C) Secondary analysis of lead candidate siRNAs selected from the primary RNAi screen of genes that regulate epigenetic processes. The nuclear YFP intensity data are presented relative to that of cells transfected with a scrambled siRNA (siNeg.1). The results from siHIRA.2 are shown as a positive control.