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. 2023 Jun 6;14:1181499. doi: 10.3389/fimmu.2023.1181499

Table 2.

LncRNAs regulating CD4+ T cell subsets during homeostasis and cancer.

LncRNA LncRNA type Molecular Interaction Cancer type T cell subset Species Affected Signaling Molecules/Pathway Function Ref.
CRNDE-h intergenic, antisense lncRNA- protein CRC,
Glioma, HCC, LC
Th17 h RORγ/IL-17 CRNDE-h is a tumor exosome-transmitted lncRNA promoting Th17 cell differentiation by inhibiting Itch-mediated ubiquitination and degradation of RORγt in CRC. (99)
Dreg1 antisense Th2 h, m Dreg1/Gata3 Dreg1 induces Gata3 expression in Th2 cells. (100)
GATA3- AS1 antisense lncRNA- protein Th2 h WDR5/GATA3/IL-5/ IL-4/IL-13 GATA3-AS1, neighboring the Gata3 locus, induces Th2 lineage commitment by interacting with the methyltransferase WDR5 and inducing GATA3, IL-5 and IL-13 expression. (101)
LINC00892 intergenic, sense NHL CD4+ T cells h, m LINC00892 is induced by T cell activation and expressed in CD4+ T helper and follicular lymphoma T helper cells. (102)
LINC01882 intergenic, antisense CD4+ T cells h LINC01882/ZEB1/MA P2K4 LINC01882 is expressed in naïve CD4+ T cells. Its knockdown increased the expression of transcription factor ZEB1 and MAP2K4, both linked to IL-2 signaling. (103)
linc-Ccr2- 5′AS intergenic Th2 m Ccr1/Ccr2/Ccr3/Ccr5 Linc-Ccr2-5′AS and the transcription factor GATA3 represent a key regulatory network for Th2-specific cell differentiation and migration. (104)
linc-MAF-4 intergenic lncRNA- DNA Th1 h EZH2/LSD1/MAF-4 Linc-MAF-4 modulates Th1 differentiation and function through interaction with EZH2 and LSD1 to epigenetically repress MAF. (105)
Lnc-DDIT4 lncRNA- protein Th17 h Lnc-DDIT4/DDIT4/
mTOR signaling
Lnc-DDIT4 induces DDIT4 expression, which blocks mTOR signaling and inhibits IL-17 production. (106)
LncRNA- GAS5 sense lncRNA- protein Th17 h, m GAS5/STAT3 lncRNA-GAS5 inhibits Th17 differentiation by modulating STAT3. (107)
lncRNA- GM lncRNA- protein Th17 h, m lncRNA-GM/Foxo1 lncRNA-GM directly inhibits Foxo1 dephosphorylation, thus promoting IL-23R expression and Th17 differentiation. (108)
NEAT1 intergenic lncRNA- protein Th17 h, m NEAT1/STAT3 NEAT1 reduces STAT3 levels, thereby blocking Th17 cell differentiation in PBMCs of rheumatoid arthritis patients. (109)
NeST intergenic, antisense lncRNA- protein CD8+ T cells, CD4+ Th1 h, m NeST/WDR5 NeST binds to WDR5, a component of the H3K4 methyltransferase complex, thus increasing H3K4me3 at the IFN- γ locus in CD8+ and CD4+ Th1 cells. (77, 78)
NKILA sense lncRNA- protein BC, LC Th1,
CD8+ T cells
h Ca2+/ calmodulin/
NFκB/STAT1
NKILA regulates T cell sensitivity to AICD by inhibiting NFkB activity and its anti-apoptotic target genes via IkBa. (28)
NNT intergenic, antisense CD4+ T cells h IFN-γ signaling NNT is expressed in activated CD4+ T cells and is localized near the IFN-γ receptor gene, indicating a functional relationship. (110)
NRON intronic, sense lncRNA- protein CD8+ T cells, CD4+ T cells h, m NRON/NFAT-IQGAP- CK1-GSK3-DYRK NRON (noncoding repressor of NFAT) regulates nuclear trafficking of NFAT via an RNA-protein scaffold complex to limit excessive T cell activation and NFAT-dependent cytokine production. (7981)
RUNX1-IT1 intronic, antisense lncRNA- protein Th1 h RUNX1-
IT1/p53/NrCAM
RUNX1-IT1 regulates NrCAM through interacting with p53, thereby modulating T-bet and CXCL10 levels in Graves’ disease. (111)
STAT4-AS1 sense lncRNA- protein Th17 h STAT4-AS1/ROR-γt STAT4-AS1 inhibits Th17 differentiation by binding ROR-γt and blocking its translocation to the nucleus and its binding to the IL-17 promoter. (112)
Th2-LCR
lncRNA cluster
antisense, sense lncRNA- DNA Th2 h, m MLL/IL-4/IL-5/IL-13 The Th2-LCR lncRNA cluster regulates through histone acetylation, (de-)methylation and chromatin remodeling the Th2 cytokine locus including IFN-γ, IL-4, IL-5 and IL-13. (56, 113, 114)
Xloc003810 MG-T Th17,
Treg cells
h Xloc003810/IFN-γ/ TNF-α/IL-1β Xloc003810 increases thymic Th17/Treg ratio and CD4+ PD1+ T cells in MG-T, which is accompanied by increased production of IFN-γ, TNF-α and IL-1β. (115,
116)

BC, breast cancer; CRC, colorectal cancer; HCC, hepatocellular carcinoma; LC, lung cancer; MG-T, myasthenia gravis with thymoma; NHL, non-Hodgkin B-cell lymphoma; h, human; m, mouse.