Figure 3.

LOXL2 promoted the cell viability of CRC cells treated with 5-FU. The scramble and shLOXL2 vector were transfected into SW48 cells, while HCT116 cells were transfected with LOXL2 expression and empty vector. (A) The LOXL2 overexpression in HCT116 cell line and knockdown efficiencies in SW48 cell line were validated by Western blotting. (B) The cell abilities were measured by CCK8 assays. The IC50 values of 5-FU for HCT116/Vector, HCT116/LOXL2, SW48/Scamble, SW48/shLOXL2-1, and SW48/shLOXL2-2 cells were 16.85, 24.29, 19.22, 11.47, and 10.81 μM, respectively.

ns: not significant.

**p < 0.01, *p < 0.05.