Fig 6. ARID1A is critical for Trm formation.

(A) PageRank and mRNA expression analysis of Arid1a^cKO and WT MP cells from d8 p.i. (B) Absolute numbers of WT (n=8) and Arid1a^cKO (n=8) P14 splenic memory cells at d30-d60 p.i. (C) Representative cytokine production in WT (n=4) and Arid1a^cKO (n=4) memory P14 cells in the spleen at d60 p.i. Mean frequency of IFNg⁺TNF⁺ (top) and IFNg⁺CCL3⁺ (bottom) populations are shown. (D) Frequency of KLRG1⁺CD127⁻ or KLRG1⁺CX₃CR1⁺ secondary effector cells 8d p.i. following LM-GP33 infection in mice that previously received CD127⁺ WT and Arid1a^cKO LCMV memory P14 cells. (E) TCF1 staining in WT and Arid1a^cKO CD127^hi spleen memory cells at d50 p.i. (F) Absolute numbers of WT and Arid1a^cKO P14 cells in the liver (n=7) and SI-IEL (n=7) at d50-d60 p.i. (G) Representative flow cytometry plots of WT and Arid1a^cKO P14 SI-IELs at d60 p.i. Mean frequency of CD69⁺CD103⁺ (top) and CD49a⁺CD103⁺ (bottom) populations are shown. Granzyme B (H) and TCF1 (I) staining in WT and Arid1a^cKO P14 SI-IELs at d60 p.i. Paired t-test; *p<0.05, **p<0.005, ***p<0.0005.