Fig 3. Toxin sensitive prey growth restriction is dependent on the type of toxin and dose delivered.
(A, B) Representative image of 48h old mixed colonies of toxin sensitised bacteria in red in competition with T6SS+ or T6SS- (ΔtssB1ΔtssB2ΔtssB3) strains of the same regulatory background in green. Upper lane contains a control mix of bacteria with full toxin-immunity gene sets, each of the following lanes contains strain sensitised to one of the H1-T6SS toxins from Tse1 to Tse8. Images sets of competitions of ΔrsmA (A) and ΔretS (B) regulatory background strains shown with each of the sets containing both single fluorescence channel and overlay images showing distribution of sensitised prey in a mix with T6SS+ and subsequently T6SS- (ΔtssB1ΔtssB2ΔtssB3) parental strain. Strains contain constitutively expressed fluorescent proteins, prey labelled with mCherry (shown in red) and attacker with sfGFP (shown in green). All bacteria mixed at 1:1 ratio, inoculum OD600 = 1.0, grown for 48h at 37°C on LB with 2% (w/v) agar.