Fig. 3.

Decellularized lung composition characterization. A. Collagen to tissue weight ratio was increased with decellularization, mostly due to cell removal. B. Collagen I and collagen IV staining showed preservation of both collagen types and preservation of the overall structure. C. Picro-sirius red staining showed that collagen was preserved through decellularization D. Quantification of GAGs showed a reduction after decellularization. E. Alcian blue staining showed preservation of the distribution of GAGs. F. Quantification of elastin abundance showed a reduction after decellularization. G. Elastic van Gieson staining showed that elastin structure was preserved throughout the lung. H. Mucins, elastic and reticular fibres are preserved as assessed by Masson's trichrome and MOVAT staining I. Laminin immunofluorescence (IF) staining showed preservation after decellularization. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)