Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-mouse CD44 (IM7) | Leinco | Cat# C382 RRID: AB_2737485 |
| anti-mouse GITR (DTA-1) | BioXcell | Cat# BE0063 RRID: AB_1107688 |
| anti-mouse CD25 (PC61) | Leinco | Cat# C1194 RRID: AB_2737451 |
| anti-mouse CD38 (90) | eBioscience | Cat# 14-0381-82 RRID: AB_467219 |
| anti-mouse CD90 (G7) | Biolegend | Cat# 105202 RRID: AB_313169 |
| anti-mouse Lag-3 (C9B7W) | Leinco | Cat# L306 RRID: AB_2737552 |
| anti-mouse CD27 (LG.7F9) | eBioscience | Cat# 50-124-94 |
| anti-mouse KLRG1 (2F1/KLRG1) | BioXCell | Cat# BE0201 RRID: AB_10949054 |
| anti-mouse CD103 (2E7) | Biolegend | Cat# 121402 RRID: AB_535945 |
| anti-mouse CD4 (GK1.5) | BioXcell | Cat# BE0003-1 RRID: AB_1107636 |
| anti-mouse CD45 (30-F11) | Fluidigm | Cat# 3089005B RRID: AB_2651152 |
| anti-mouse CD62L (MEL-14) | Leinco | Cat# C2118 RRID: AB_2737457 |
| anti-mouse ICOS (C398.4A) | eBioscience | Cat# 14-9949-82 RRID: AB_468637 |
| anti-mouse OX-40 (OX-86) | BioXcell | Cat# BE0031 RRID: AB_1107592 |
| anti-mouse PD-1 (RMP1-30) | eBioscience | Cat# 14-9981-82 RRID: AB_468655 |
| anti-mouse TIGIT (1G9) | BioXcell | Cat# BE0274 RRID: AB_2687797 |
| anti-mouse CD69 (H1.2F3) | eBioscience | Cat# 14-0691-82 RRID: AB_467325 |
| anti-mouse TCRβ (H57-597) | BioXcell | Cat# BE0102 RRID: AB_10950158 |
| anti-mouse CD127 (A7R34) | BioXcell | Cat# BE0065 RRID: AB_1107590 |
| anti-mouse CD39 (Duha59) | Biolegend | Cat# 143802 RRID: AB_11204243 |
| anti-mouse NK1.1 (PK136) | BioXcell | Cat# BE0036 RRID: AB_1107737 |
| anti-mouse CD8α (53-6.7) | Leinco | Cat# C375 RRID: AB_2737478 |
| anti-mouse TCRγδ (GL3) | eBioscience | Cat# 14-5711-82 RRID: AB_467569 |
| anti-mouse Tim3 (RMT3-23) | BioXcell | Cat# BE0115 RRID: AB_10949464 |
| anti-mouse FoxP3 (FJK-16s) | eBioscience | Cat# 14-5773-82 RRID: AB_467576 |
| anti-mouse GATA3 (TWAJ) | eBioscience | Cat# 14-9966-82 RRID: AB_1210519 |
| anti-mouse GranzymeB (GB11) | eBioscience | Cat# MA1-80734 RRID: AB_931084 |
| anti-mouse CTLA-4 (UC10-4B9) | eBioscience | Cat# 50-129-16 |
| anti-mouse Ki67 (8D5) | Novus | Cat# NBP2-22112 |
| anti-mouse TCF1 (812145) | R&D | Cat# MAB8224 |
| anti-mouse ROR-γt (AFKJS-9) | eBioscience | Cat# 14-6988-82 RRID: AB_1834475 |
| anti-mouse Eomes (Dan11mag) | eBioscience | Cat# 50-245-556 |
| anti-mouse T-bet (4B10) | Biolegend | Cat# 644802 RRID: AB_1595503 |
| anti-mouse CD45 (30-F11) | Fluidigm | Cat# 3089005B RRID: AB_2651152 |
| anti-mouse Ki67 (8D5) | Novus | Cat# NBP2-22112 |
| anti-mouse CD11c (N418) | Fluidigm | Cat# 3142003B RRID: AB_2814737 |
| anti-mouse CD68 (FA-11) | Biolegend | Cat# 137001 RRID: AB_2044003 |
| anti-mouse MHC-I (28-14-8) | Fluidigm | Cat# 3144016B RRID: AB_2687831 |
| anti-mouse CD206 (C068C2) | Biolegend | Cat# 141702 RRID: AB_10900233 |
| anti-mouse F4/80 (BM8) | Fluidigm | Cat# 3146008B RRID: AB_2895117 |
| anti-mouse MHC-II (M5/114.15.2) | Biolegend | Cat# 107602 RRID: AB_313317 |
| anti-mouse CD11b (M1/70) | Fluidigm | Cat# 3148003B RRID: AB_2814738 |
| anti-mouse CD172α/SIRPα (P84) | Biolegend | Cat# 144002 RRID: AB_11203711 |
| anti-mouse Ly6C (HK1.4) | Fluidigm | Cat# 3150010B RRID: AB_2895118 |
| anti-mouse Ly6G (1A8) | Fluidigm | Cat# 3151010B |
| anti-mouse CD64 (X54-5/7.1) | Biolegend | Cat# 139301 RRID: AB_10612757 |
| anti-mouse Tim4 (RMT4-54) | Biolegend | Cat# 130002 RRID: AB_1227802 |
| anti-mouse XCR1(Zet) | Biolegend | Cat# 148202 RRID: AB_2563841 |
| anti-mouse CD103 (2E7) | Biolegend | Cat# 121402 RRID: AB_535945 |
| anti-mouse NK1.1 (PK136) | BioXcell | Cat# BE0036 RRID: AB_1107737 |
| anti-mouse Bst2 (120G8) | Novous/imgenx | Cat# DDX0390P-100 RRID: AB_2827525 |
| anti-mouse IRF4 (3E4) | Biolegend | Cat# 646402 RRID: AB_2280462 |
| anti-mouse CD39 (Duha59) | Biolegend | Cat# 143802 RRID: AB_11204243 |
| anti-mouse NK1.1 (PK136) | BioXcell | Cat# BE0036 RRID: AB_1107737 |
| anti-mouse CD83 (Michel-17) | thermofisher scientific | Cat# 14-0831-82 RRID: AB_467357 |
| anti-mouse CD40 (HM40-3) | Fluidigm | Cat# 124601 RRID: AB_1134239 |
| anti-mouse Ox40L (RM134L) | Biolegend | Cat# 108802 RRID: AB_313401 |
| anti-mouse CCR2 (475301) | R&D systems | Cat# MAB55381-100 RRID: AB_2749828 |
| anti-mouse Cx3CR1(SA011F11) | Fluidigm | Cat# 3164023B RRID: AB_2832247 |
| anti-mouse CCR7 (4B12) | Biolegend | Cat# 120101 RRID: AB_389229 |
| anti-mouse PDL2 (TY25) | BioXCell | Cat# BE0112 RRID: AB_10950106 |
| anti-mouse VISTA (MIH63) | Biolegend | Cat# 150202 RRID: AB_2565897 |
| anti-mouse Tim3 (RMT3-23) | BioXcell | Cat# BE0115 RRID: AB_10949464 |
| anti-mouse PD-L1 (10F.9G2) | BioXCell | Cat# BE0101 RRID: AB_10949073 |
| anti-mouse CD80 (16-10A1) | Biolegend | Cat# 104702 RRID: AB_313123 |
| anti-mouse CD135/FLT3 (A2F10) | Thermo Fisher Scientific | Cat# 14-1351-82 RRID: AB_467481 |
| anti-mouse CD86 (GL1) | Fluidigm | Cat# 3172016B RRID: AB_2922923 |
| anti-mouse B220 (RA3-682) | Fluidigm | Cat# 3144011B RRID: AB_2811239 |
| anti-mouse CD45 (30-F11) | eBioscience | Cat# 25-0451-82 RRID: AB_2734986 |
| anti-mouse CD45 (30-F11) | BD Biosciences | Cat# 564225 RRID: AB_2716861 |
| anti-mouse CD45 (30-F11) | BioLegend | Cat# 103184 |
| anti-mouse CD45 (30-F11) | Invitrogen | Cat# 63-045-182 |
| anti-mouse CD3e (145-2C11) | eBioscience | Cat# 17-0031-82 RRID: AB_469315 |
| anti-mouse CD3e (145-2C11) | BD Biosciences | Cat# 563123 RRID: AB_2687954 |
| anti-mouse CD4 (RM4-4) | eBioscience | Cat# 11-0043-82 RRID: AB_464900 |
| anti-mouse CD4 (RM4-4) | BioLegend | Cat# 116022 RRID: AB_2715958 |
| anti-mouse CD8α (53-6.7) | BD Biosciences | Cat# 563786 RRID: AB_2732919 |
| anti-mouse CD8α (53-6.7) | BD Biosciences | Cat# 553035 RRID: AB_398527 |
| anti-mouse Foxp3 (FJK-16s) | eBioscience | Cat# 15-5773-82 RRID: AB_468806 |
| anti-mouse CD19 (eBio1D3) | eBioscience | Cat# 17-0193-82 RRID: AB_1659676 |
| anti-mouse CD11b (M1/70) | eBioscience | Cat# 56-0112-82 RRID: AB_657585 |
| anti-mouse CD11b (M1/70) | Invitrogen | Cat# 56-0112-82 RRID: AB_657585 |
| anti-mouse Ly6C (HK1.4) | eBioscience | Cat# 45-5932-82 RRID: AB_2723343 |
| anti-mouse Ly6C (HK1.4) | BioLegend | Cat# 128006 RRID: AB_1186135 |
| anti-mouse Ly6G (1A8) | BioLegend | Cat# 127608 RRID: AB_1186099 |
| anti-mouse Ly6G (1A8) | BioLegend | Cat# 127614 RRID: AB_2227348 |
| anti-mouse F4/80 (BM8) | eBioscience | Cat# 15-4801-82 RRID: AB_468798 |
| anti-mouse F4/80 (BM8) | Invitrogen | Cat# 25-4801-82 RRID: AB_469653 |
| anti-mouse MHC-II (M5/115.15.2) | eBioscience | Cat# 48-5321-82 RRID: AB_1272204 |
| anti-mouse MHC-II (I-A/I-E) (M5/114.15.2) | Invitrogen | Cat# 58-5321-82 RRID: AB_2811913 |
| anti-mouse Ki67 (SolA15) | eBioscience | Cat# 50-5698-82 RRID: AB_2574235 |
| anti-mouse SIINFEKL Dextramer (N/A) | Immudex | Cat# JD2163 |
| anti-mouse Granzyme B (GB11) | BD Horizon | Cat# 563388 RRID: AB_2738174 |
| anti-mouse IFNγ (XMG1.2) | BioLegend | Cat# 505808 RRID: AB_315402 |
| anti-mouse TNFα (MP6-XT22) | eBioscience | Cat# 11-7321-82 RRID: AB_465418 |
| CD11b (EPR1344) | Abcam | Cat# ab13357 RRID: AB_2650514 |
| F4/80 (D2S9R) | Cell Signaling | Cat# 70076 RRID: AB_2799771 |
| STING (D2P2F) | Cell Signaling | Cat# 13647 RRID: AB_2732796 |
| NFκB p65 (D14E12) | Cell Signaling | Cat# 8242 RRID: AB_10859369 |
| pSTAT1 (D3B7) | Cell Signaling | Cat# 8826 RRID: AB_2773718 |
| CK19 (Troma III) | DSHB | Cat# Uoflowa DSHB TROMA-III C |
| CD8 (D4W2Z) | Cell Signaling | Cat# 98941 RRID: AB_2756376 |
| CD8 (SP16) | Invitrogen | Cat# MA5-14548 RRID: AB_10984334 |
| CD163 (10D6) | Leica Biosystem | Cat# NCL-L-CD163 RRID: AB_2756375 |
| Keratin17/19 (D4G2) | Cell Signaling | Cat# 12434 RRID: AB_2797912 |
| Cytokeratin, Muti (AE1/AE3) | Leica Biosystem | Cat# NCL-L-AE1/AE3-601 RRID: AB_2924990 |
| pAKT (D9E) | Cell Signaling | Cat# 4060 RRID: AB_2315049 |
| pIRF3 (D6O1M) | Cell Signaling | Cat# 29047 RRID: AB_2773013 |
| IRF3 (D83B9) | Cell Signaling | Cat# 4302 |
| STAT1 (D1K9Y) | Cell Signaling | Cat# 14994 RRID: AB_2737027 |
| pFAK | Cell Signaling | Cat# 3283 RRID: AB_2173659 |
| FAK | Cell Signaling | Cat# 3285 RRID: AB_2269034 |
| pp65 (93H1) | Cell Signaling | Cat# 3033 RRID: AB_331284 |
| pIκBα (14D4) | Cell Signaling | Cat# 2859 RRID: AB_561111 |
| IκBα | Cell Signaling | Cat# 9242 RRID: AB_331623 |
| Cleaved Caspase-3 (Asp175) | Cell Signaling | Cat# 9661 RRID: AB_2341188 |
| Ubiquitin (E4I2J) | Cell Signaling | Cat# 43124 RRID: AB_2799235 |
| SIRT3 (D22A3) | Cell Signaling | Cat# 5490 RRID: AB_10828246 |
| β-ACTIN (13E5) | Cell Signaling | Cat# 4970 RRID: AB_2223172 |
| IFNβ | Invitrogen | Cat# PA5-20390 RRID: AB_11155641 |
| CXCL10 (10H11L3) | Invitrogen | Cat# 701225 RRID: AB_2532429 |
| Oligonucleotides | ||
| Primers and siRNA used in this study are list in Table S6 | This paper | N/A |
| Biological Samples | ||
| Human PDAC TMA | Washington University | IRB# 201704078 |
| Tumor biopsies | Gossamer Bio | # NTC04060342 |
| Cultrex Basement membrane extract, Pathclear | Trevigen | Cat# 3432-001-01 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| STING agonist (ML-RR-S2 CDA; ADU-S100) | MedChemExpress | Cat# HY-12885B |
| Gemcitabine hydrochloride | Ark Pharm | Cat# 122111-03-9 |
| Paclitaxel | Fresenius Kabi | #00363323763503 |
| InVivoMAb anti-mouse IL-1R (CD121a) (JAMA-147) | BioXCell | Cat# BE0256 |
| InVivoMAb anti-mouse CD4 (GK1.5) | BioXCell | Cat# BE0003-1 |
| InVivoMAb anti-mouse CD8 (2.43) | BioXCell | Cat# BP0061 |
| ODN 1585 -TLR9 ligand | InvivoGen | Cat# tlrl-1585 |
| R848 (Resiquimod) | InvivoGen | Cat# tlrl-r848-5 |
| Autophagy inhibitor, 3-MA | Sigma-Aldrich | Cat# 5142-23-4 |
| N-acetyl-L-cysteine | Sigma-Aldrich | Cat# 616-91-1 |
| 2'3'- ethidium bromide solution | Invitrogen | Cat# 17898 |
| MG132 | MedChemExpress | Cat# HY-13259 |
| Collagenase A | Sigma/Roche | Cat# 10103586001 |
| DNAse I | Sigma | Cat# 11284932001 |
| Cell Stimulation cocktail (PMA/Iono) | Ebioscience | Cat# 00-4970-93 |
| Brefeldin A | Biolegend | Cat# 420601 |
| Monensin | Biolegend | Cat# 420701 |
| 2′-7′-dichlorodihydrofluorescein diacetate | Sigma-Aldrich | Cat# 287810 |
| Critical Commercial Assays | ||
| E.Z.N.A. Total RNA kit I | Omega | Cat# R6834-02 |
| qScript cDNA Supermix kit | Quantabio | Cat# 95048-500 |
| Taqman Gene Expression Master Mix | Thermo Fisher | Cat# 4370074 |
| BOND Polymer Refine Detection kit Leica #DS9800 | Leica | Cat# DS9800 |
| BOND Intense R Detection kit | Leica | Cat# DS9263 |
| Cytofix kit | BD Bioscience | Cat# 554655 |
| CSF1 neutralizing antibody (Clone 5A1) | BioXCell | Cat# BE0204 |
| PBS Liposomes & Clodronate Liposomes | Liposoma | Cat# CP-005-005 |
| Halt Protease and Phosphatase Inhibitor | Thermo Scientific | Cat# 78442 |
| RIPA Buffer(10x) | Cell Signaling | Cat# UN3082 |
| Mouse Macrophage Nucleofector Kit | Lonza | Cat# VPA-1009 |
| Pierce BCA Protein Assay Kit | Thermo Scientific | Cat# 23225 |
| Recombinant Murine M-CSF | PeproTech | Cat# 315-02 |
| Mouse IFN-beta DuoSet ELISA | R&D Systems | Cat# DY8234-05 |
| Mouse IL-1 beta/IL-1F2 DuoSet ELISA | R&D Systems | Cat# DY401 |
| IP-10/CXCL10 Mouse Matched Antibody Pair | Invitrogen | Cat# BMS6018MST |
| I-TAC/CXCL11 Mouse ELISA Kit | Invitrogen | Cat# EMCXCL11 |
| protein A agarose Beads | Cell Signaling Technology | Cat# 9863 |
| NucleoSpin Tissue Kit | Takara Bio | Cat# 740952.50 |
| SuperSignal™ West Dura Extended Duration Substrate | Thermo Fisher Scientific | Cat# 34076 |
| CellTiter96 Non-Radioactive Cell Proliferation Assay | Promega | Cat# G4002 |
| EasySep Human CD14 Selection Kit | Stemcell Technologies | Cat# 17858 |
| Pierce™ Protein Concentrator PES, 3K MWCO | Thermo Fisher Scientific | Cat# 88525 |
| Experimental Models: Cell Lines | ||
| KP2/KP1; derived from a KPC tumor | This paper; Jiang et al.2016 | N/A |
| KI; derived from Kras-Ink mouse | This paper; Jiang et al.2016 | N/A |
| KP2-OVA-GFP; generated from KP2 cells transduced with OVA-GFP containing lentivirus and sorted for GFP | This paper; VE Lander et at. 2022 | N/A |
| Panc-1 | obtained from Dr. Kian H from ATCC | RRID: CVCL_0480 |
| Capan-1 | obtained from Dr. Kian H from ATCC | RRID: CVCL_0237 |
| CFPAC-1 | obtained from Dr. Kian H from ATCC | RRID: CVCL_1119 |
| HPAC | obtained from Dr. Kian H from ATCC | RRID: CVCL_3517 |
| Experimental Models: Organisms/Strains | ||
| mouse: p48-Cre;KrasLSL-G12D;Trp53fl/fl | Hingorani et al., 2003; Morton et al., 2010 | N/A |
| mouse: C57BL/6J | The Jackson Laboratory | Stock# 000664 |
| mouse: FVB/NCr | Charles River Laboratories | Strain# 559 |
| mouse: B6.129S4-Itgamtm1Myd/J | The Jackson Laboratory | Strain# 003991 |
| mouse: B6(Cg)-Sting1tm1.2Camb/J | The Jackson Laboratory | Strain# 025805 |
| mouse: C57BL/6-TIrgem1.1Ldm/j | The Jackson Laboratory | Strain# 034449 |
| Deposited Data | ||
| scRNASeq data | This paper | GSE220959 |
| Software and Algorithms | ||
| Flowjo v10.7.2 | Flowjo, L.L.C. | Flowjo, L.L.C. RRID:SCR_008520 |
| Prism v9 | Graphpad |
www.graphpad.com RRID:SCR_000306 |
| Docker | Rocker/rstudio:latest | https://hub.docker.com/r/rocker/rstudio |
| cumulusprod/cellranger:4.0.0 | https://hub.docker.com/r/cumulusprod/cellranger/tags | |
| HALO v3.2.1851 | Indica Labs-High Plex Fv4.0.3 | https://indicalab.com/products/high-plex-fl/ |
| Indica Labs-Deconvolution v1.0.4 | ||
| Cytobank | Cytobank, Inc | Wustl.cytobank.org |
| FACSDiva | BD Biosciences | RRID: SCR_001456 |
| Zen | Zeiss | Zeiss.com |
| Fiji v2.0.0 | ImageJ | |
| R v3.6.3 | R Core Team | https://cran.r-project.org/bin/windows/base/old/3.6.3/ |
| Clusterprofiler | https://github.com/YuLab-SMU/clusterProfiler | |
| Seurat v 3.2.0 | https://satijalab.org/seurat/ | |
| Harmony | https://github.com/immunogenomics/harmony | |
| CATALYST | https://github.com/HelenaLC/CATALYST | |