Figure 5.

Transient receptor potential vanilloid type 4 (TRPV4) deletion does not change epithelial Na⁺ channel (ENaC) and large-conductance K⁺ channel (BK) levels. A, top: actual Western blot probed with anti-αENaC antibodies from whole kidney lysates of TRPV4^fl/fl and TRPV4^fl/fl-Pax8Cre mice maintained on a high-K⁺ diet (5% K⁺) for 7 days. The full-length αENaC band around 100 kDa as well as a bright nonspecific band are indicated by arrows. Each line represents an individual animal (2 females and 2 males). A, bottom: Ponceau red staining of the same nitrocellulose membrane demonstrating equal protein loading. B: summary graph comparing full-length αENaC expression in TRPV4^fl/fl and TRPV4^fl/fl-Pax8Cre mice from the conditions shown in A. Intensity values were normalized to the total signal of the respective lines in Ponceau red staining. Males and females are indicated as triangles and circles, respectively. C, top: actual Western blot probed with anti-αBK antibodies from whole kidney lysates of TRPV4^fl/fl and TRPV4^fl/fl-Pax8Cre mice maintained on a high-K⁺ diet (5% K⁺) for 7 days. Each line represents an individual animal (2 females and 2 males). C, bottom: Ponceau red staining of the same nitrocellulose membrane demonstrating equal protein loading. D: summary graph comparing αBK expression in TRPV4^fl/fl and TRPV4^fl/fl-Pax8Cre mice from the conditions shown in A. Intensity values were normalized to the total signal of the respective lines in Ponceau red staining. *Significant increase (P < 0.05, one-way ANOVA with a post hoc Tukey’s test).