Figure 7.

H⁺-K⁺-ATPase levels are increased in transient receptor potential vanilloid type 4 (TRPV4)^fl/fl-Pax8Cre mice on both regular and low K⁺ intake. A, top: representative Western blot probed with anti-H⁺-K⁺-ATPase β-subunit antibodies from whole kidney lysates of TRPV4^fl/fl and TRPV4^fl/fl-Pax8Cre mice maintained on a regular-K⁺ diet (0.9% K⁺) and a low-K⁺ diet (<0.01% K⁺) for 7 days. Each line represents an individual animal. A, bottom: Ponceau red staining of the same nitrocellulose membrane demonstrating equal protein loading. B: summary graph comparing H⁺-K⁺-ATPase β-subunit expression in TRPV4^fl/fl mice (3 females and 3 males) and TRPV4^fl/fl-Pax8Cre mice (3 females and 3 males) from the conditions shown in A. Intensity values were normalized to the total signal of the respective lines in Ponceau red staining. Males and females are indicated as triangles and circles, respectively. *Significant changes (P < 0.05, one-way ANOVA with a post hoc Tukey’s test) vs. the respective values of TRPV4^fl/fl mice, as indicated by the horizontal bars.