Table 7.
Muscle fatty acid composition of juvenile mud crab Scylla paramamosain fed with different dietary ARA levels (mg g−1 dry matter).
| Fatty acids | Dietary ARA levels (g kg−1) | ANOVA | Linear | Quadratic | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.40 | 2.50 | 4.60 | 8.90 | 12.50 | 15.70 | p value | p value | R 2 | p value | R 2 | |
| 14 : 0 | 0.11 ± 0.01 | 0.11 ± 0.01 | 0.09 ± 0.01 | 0.09 ± 0.00 | 0.08 ± 0.00 | 0.10 ± 0.01 | 0.135 | 0.097 | 0.111 | 0.044 | 0.252 |
| 16 : 0 | 2.49 ± 0.04b | 2.92 ± 0.02d | 2.68 ± 0.05c | 1.93 ± 0.03a | 2.00 ± 0.01a | 1.96 ± 0.00a | 0.000 | 0.000 | 0.637 | 0.000 | 0.615 |
| 18 : 0 | 1.35 ± 0.04b | 1.18 ± 0.01a | 1.26 ± 0.01ab | 1.36 ± 0.01b | 1.38 ± 0.06b | 1.33 ± 0.01b | 0.003 | 0.103 | 0.105 | 0.272 | 0.047 |
| ∑SFAa | 3.95 ± 0.01b | 4.21 ± 0.02c | 4.03 ± 0.05b | 3.38 ± 0.03a | 3.47 ± 0.05a | 3.38 ± 0.02a | 0.000 | 0.000 | 0.711 | 0.000 | 0.698 |
| 16:1n-7 | 0.30 ± 0.02d | 0.26 ± 0.00cd | 0.22 ± 0.00bc | 0.19 ± 0.01ab | 0.16 ± 0.01ab | 0.16 ± 0.01a | 0.000 | 0.000 | 0.808 | 0.000 | 0.881 |
| 18:1n-9 | 1.75 ± 0.00c | 1.76 ± 0.00c | 1.62 ± 0.00b | 1.53 ± 0.03a | 1.52 ± 0.04a | 1.62 ± 0.00b | 0.000 | 0.001 | 0.449 | 0.000 | 0.769 |
| ∑MUFAb | 2.05 ± 0.02c | 2.02 ± 0.00c | 1.85 ± 0.00b | 1.72 ± 0.04a | 1.68 ± 0.03a | 1.79 ± 0.01ab | 0.000 | 0.000 | 0.632 | 0.000 | 0.879 |
| 18:2n-6 | 0.42 ± 0.03 | 0.40 ± 0.01 | 0.39 ± 0.01 | 0.40 ± 0.03 | 0.40 ± 0.03 | 0.34 ± 0.01 | 0.214 | 0.047 | 0.176 | 0.107 | 0.159 |
| 20:4n-6 | 0.41 ± 0.01a | 0.78 ± 0.01b | 1.02 ± 0.00c | 1.11 ± 0.04c | 1.44 ± 0.07d | 1.88 ± 0.02e | 0.000 | 0.000 | 0.929 | 0.000 | 0.925 |
| ∑n − 6PUFAc | 0.83 ± 0.03a | 1.18 ± 0.01b | 1.41 ± 0.01c | 1.51 ± 0.06c | 1.84 ± 0.09d | 2.23 ± 0.03e | 0.000 | 0.000 | 0.927 | 0.000 | 0.922 |
| 18:3n-3 | 0.06 ± 0.00a | 0.08 ± 0.00b | 0.06 ± 0.00a | 0.05 ± 0.00a | 0.05 ± 0.01a | 0.05 ± 0.01a | 0.000 | 0.002 | 0.411 | 0.011 | 0.378 |
| 20:5n-3 | 2.21 ± 0.01e | 2.03 ± 0.01c | 1.92 ± 0.01b | 2.08 ± 0.03cd | 2.15 ± 0.01de | 1.53 ± 0.00a | 0.000 | 0.010 | 0.311 | 0.008 | 0.401 |
| 22:5n-3 | 0.12 ± 0.01c | 0.12 ± 0.01bc | 0.10 ± 0.00abc | 0.09 ± 0.00ab | 0.10 ± 0.00abc | 0.08 ± 0.01a | 0.001 | 0.000 | 0.605 | 0.001 | 0.588 |
| 22:6n-3 | 1.79 ± 0.04c | 1.62 ± 0.01b | 1.50 ± 0.01a | 1.52 ± 0.03ab | 1.62 ± 0.01b | 1.55 ± 0.03ab | 0.000 | 0.050 | 0.170 | 0.002 | 0.506 |
| ∑n − 3PUFAd | 4.18 ± 0.05e | 3.85 ± 0.03cd | 3.58 ± 0.02b | 3.75 ± 0.01c | 3.92 ± 0.02d | 3.22 ± 0.03a | 0.000 | 0.002 | 0.415 | 0.011 | 0.378 |
| ∑n − 3LC − PUFAe | 4.12 ± 0.05e | 3.77 ± 0.03cd | 3.52 ± 0.01b | 3.70 ± 0.01c | 3.87 ± 0.01d | 3.17 ± 0.03a | 0.000 | 0.004 | 0.384 | 0.016 | 0.346 |
| ARA/EPAf | 0.19 ± 0.00a | 0.38 ± 0.00b | 0.54 ± 0.00c | 0.53 ± 0.03c | 0.67 ± 0.03d | 1.23 ± 0.01e | 0.000 | 0.000 | 0.815 | 0.000 | 0.852 |
| ARA/DHAg | 0.23 ± 0.01a | 0.48 ± 0.01b | 0.68 ± 0.01c | 0.73 ± 0.03c | 0.89 ± 0.05d | 1.21 ± 0.03e | 0.000 | 0.000 | 0.907 | 0.000 | 0.902 |
Data are presented as mean ± SD (n = 3). Values in the same row with different superscripts are significantly different (p < 0.05). Some fatty acids, of which the contents are minor, trace amount or not detected, such as 12 : 0, 20 : 0, 22 : 0, 24 : 0, 22:1n-9, 22:1n-11, 20:2n-6, 20:3n-6, 22:4n-6, 20:5n-6, and 20:3n-3, were not listed in this table. aSFA: saturated fatty acids; bMUFA: monounsaturated fatty acids; cn-6 PUFA: n-6 polyunsaturated fatty acids; dn-3 PUFA: n-3 polyunsaturated fatty acids; en-3 LC-PUFA: n-3 long chain poly-unsaturated fatty acid; fARA/EPA, the ratio of ARA to EPA; and gARA/DHA, the ratio of ARA to DHA.