Figure 6 .

Cystamine (a PDI inhibitor) protects HT22 cells against erastin-induced ferroptosis

(A,B) Levels of dimer and monomer nNOS (A) and total nNOS level (B) after treatment with 1 μM erastin± 100 μM cystamine for 8 h. (C‒F) Levels of NO (C,D) and ROS (E,F) after treatment with 1 μM erastin± 100 μM cystamine for 8 h: fluorescence microscopy images (C,E) and quantitative intensity values (D,F). Scale bar: 45 μm. (G‒K) Levels of ROS (G,H) and lipid ROS (I,J) after treatment with 1 μM erastin± 100 μM cystamine for 8 h: flow cytometry data (G,I), quantitative intensity values (H,J), and confocal microscopy data (K). Scale bar: 50 μm. (L) Cell viability change after treatment with 1 μM erastin± 100 μM cystamine for 24 h. Data are presented as the mean±SE. n=3. * P<0.05, ** P<0.01 vs the control group.