Fig. 2. High SLC7A11 expression leads to marked increases in intracellular disulfide molecules under H₂O₂ treatment.

a–e Measurement of intracellular concentrations of cystine (a), cysteine (b), GSH (c), GSSG (d), and the GSSG/GSH ratio (e) in SLC7A11-low, -moderate, and -high 786-O cells treated with vehicle or 1 mM H₂O₂ for 2 h. f, g Relative levels of intracellular γ-glutamyl-cystine (f) and γ-glutathionyl-cysteine (g) in SLC7A11-low, -moderate, and -high 786-O cells treated with vehicle or 1 mM H₂O₂ for 2 h. h, i Measurement of intracellular concentrations of cystine (h) and GSSG (i) in T98G cells treated with vehicle or 1 mM H₂O₂ for 8 h. j Relative levels of intracellular γ-glutathionyl-cysteine in T98G cells treated with vehicle or 1 mM H₂O₂ for 8 h. Data were presented as mean ± SD; n  =  3. n indicates independent repeats. P value was determined by two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.