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. 2023 Jun 20;14:3659. doi: 10.1038/s41467-023-39436-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a 10 mice were infected with 20 or 100 tachyzoites of the indicated strain and survival monitored over the course of the experiment. p values from Log-rank (Mantel–Cox) test. b mNeon and ΔVIT parasites were used to infect macrophages at an MOI of 5 and fluorescence (arbitrary units) monitored after 72 h. Results are the mean of n = 4 independent biological replicates, performed in triplicate, ±SD. p values from two tailed t test. c After normalisation of the fluorescence in the unstimulated macrophages (from b) there was a significant decrease in survival of the ΔVIT parasites in stimulated (IFNy/LPS) macrophages. Results are the mean of n = 4 independent biological replicates, performed in triplicate, ±SD. p values from two tailed t test.