Skip to main content
. 2023 Jun 20;14:3669. doi: 10.1038/s41467-023-39403-7

Fig. 4. Turicibacter isolates differ in their bile-modifying abilities.

Fig. 4

a Schematic for types of bile transformations found to be performed by Turicibacter isolates. b inset: 16S rRNA gene-based phylogenic tree from Fig. 1a. Liquid chromatograms of individual Turicibacter isolates grown for 24 h in media with sub-inhibitory concentrations of five bile acids: taurocholic acid (TCA), cholic acid (CA), glycochenodeoxycholic acid (GCDCA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA). Shaded regions indicate expected retention time of each bile species. c Percent remaining (compared to cultures at time = 0) of conjugated bile acids (TCA, taurochenodeoxycholic acid [TCDCA], glycocholic acid [GCA], GCDCA) after 24 growth with noted Turicibacter isolate. Yellow = glycine-conjugated bile acids, orange=taurine-conjugated bile acids. n = 4 independent cultures. Values not shown were below 0.1% remaining. Statistical analysis performed by one sample t-test, annotations of legend denotes strains with significant difference from 100% remaining for each bile acid. p-values for each bile acid (MOL361/1E2/H121): TCA = (< 0.0001/ < 0.0001/0.6327); TCDCA = (< 0.0001/ < 0.0001/0.5410); GCA = (0.0002/0.6348/ < 0.0001); GCDCA = (< 0.0001/0.8803/ < 0.0001). Data are provided as source data file.