Fig. 2.

Dectin-1 deficiency ameliorated renal fibrosis induced by Ang II. A The serum creatinine levels in mice of each group. B The serum BUN levels in mice of each group. C Representative H&E staining of kidney tissues showing the effect of Dectin-1 deficiency on Ang II-induced kidney injury. D Fibrosis in kidney tissues of Ang II-challenged mice. SR panel shows representative micrographs of Sirius Red staining and Masson panel shows representative micrographs of Masson Trichrome staining. (n = 5–7; scale bar, 100 μm); E, F Quantification of interstitial fibrotic areas (%) from Sirius Red-stained kidney sections (E) and Masson’s Trichome staining (F); G Real-time qPCR showing mRNA levels of Col1a1, Col4a2, Tgfb1 and Acta2 in the kidney tissues; H Representative western blot analysis of COL4A2, COL1A1, TGF-β1 and α-SMA in kidney tissues. GAPDH was used as loading control; I Densitometric quantification of immunoblots in Fig. 2H. Levels of COL4A2, COL1A1, TGF-β1 and α-SMA were normalized to GAPDH; [A-I, n = 5–7; 1-way ANOVA followed by Tukey post-hoc tests, (B-D, F-J: number of comparisons = 10). *P < 0.05, **P < 0.01, and ***P < 0.001]