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. 2023 Jun 20;80(7):184. doi: 10.1007/s00018-023-04826-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Dectin-1 deficiency alleviated macrophage migration, neutrophil infiltration and inflammation induced by Ang II infusion. A Representative western blot analysis of G-CSF, CXCL1, and CXCR2 in kidney tissues. GAPDH was used as loading control; B Real-time qPCR showing mRNA levels of Cxcl1, Cxcr2 and Gcsf in the kidney tissues; C Real-time qPCR showing mRNA levels of Il17 and Il23 in the kidney tissues; D Representative immunohistochemical analyses of F4/80 (upper) and immunofluorescence staining of CD68 (down) in murine kidney of each group (n = 5; scale bar, 100 μm); E Representative immunofluorescence staining of Ly-6G in murine kidney of each group (n = 5; scale bar, 50 μm); F Representative immunohistochemical analyses of TNFα in murine kidney of each group (n = 5; scale bar, 100 μm); G Real-time qPCR showing mRNA levels of Tnfa, Il1b and Il6 in the kidney tissues; H Representative western blot analysis of P-Syk, Syk and IκBα in kidney tissues. GAPDH was used as loading control; [A-F, n = 5–7; 1-way ANOVA followed by Tukey post-hoc tests, (A-F: number of comparisons = 10). *P < 0.05, **P < 0.01, and ***P < 0.001]