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. 2023 Jun 20;14:3648. doi: 10.1038/s41467-023-39412-6

Fig. 5. Nrf2 mediates the protection of APAP-induced liver injury afforded by Usp25-deficiency.

Fig. 5

a Schematic of the experiment. Usp25 WT or Usp25 KO mice were injected with AdshNC or AdshNRF2 via tail vein, followed by administration of 250 mg/kg APAP for 24 h. b Western blotting analysis of the indicated proteins in the liver. Samples from 3 animals are shown. The numbers are normalized relative expression of KEAP1 and NRF2. c, d Determination of serum ALT, AST (c) levels and liver GSH (d) levels. n = 6 mice per group. e Analysis of APAP-induced necrosis in the liver. The liver sections were stained with hematoxylin and eosin and the necrotic areas were encircled and quantified. n = 6 mice per group. Scale bar, 200 μm. Error bars denote SEM. Two-tailed student’s t tests analysis (c, d and e), non-parametric tests with blue colored (c and e). Source data are provided as a Source Data file.