Fig. 7. Rescue of BBB function is associated with increased AKT phosphorylation in the vessels.

A–C Immunoblots showing protein levels of tight junction markers VE-cadherin (A), claudin-5 (B), ZO-1 (C), and loading control GAPDH in lysates extracted from the vascular compartment of BBB^G2019S chips in which the brain compartment was treated with regular growth medium or medium supplemented with 0.5 µM PD0325901 for 6 days. Protein levels are normalized to GAPDH loading control and data is shown as the fold change of treated vs. untreated BBB^G2019S vessels. D, E Immunoblots reporting protein levels of phosphorylated AKT, total AKT (D), phosphorylated p38MAPK, total p38 MAPK (E), or loading control GAPDH in lysates extracted from the vascular compartment of BBB^G2019S chips in which the brain compartment was treated with regular growth medium or medium supplemented with 0.5 µM PD0325901 for 6 days. Protein levels are normalized to GAPDH loading control and data are shown as the fold change of treated vs. untreated BBB^G2019S vessels. Data are from three biological replicates; error bars represent mean + SEM. Statistical analysis was performed using two-tailed unpaired Student’s t test with equal s.d. The BBB^G2019S nomenclature refers to the presence of LRRK2 G2019S astrocytes in the brain compartment of the BBB chip. Source data are provided as a Source Data file.