TABLE 3.

Histology stains used in the diagnosis of fungal infections (20).

Stain	Appearance of fungus in tissue	Reaction in tissue
H & E	Cytoplasm pink	Stains nucleic acids blue/purple. Stains proteins in cytoplasm, membranes, etc., pink. Useful to highlight inflammatory processes in tissue
	Nucleus blue
	Cell wall colourless
PAS	Cell wall pink to red to purple	Stains carbohydrates, polysaccharides, glycogen, mucoproteins, and mucins in tissue
GMS	Cell wall dark brown to black	Primarily used to bind to carbohydrate in cell walls of fungi. Tissue usually stained green
Fontana Masson	Stains melanin deposits brown to black	Stains melanin deposits in tissue