Table 1.

Primers used in this study, with originating loci, sequences program and references.

Gene/DNA regions	Primers				PCR amplification procedures	References
Name	Abbreviation	Name	Direction	Sequence (5’→3’)
Beta tubulin	tub	T1	Forward	AACATGCGTGAGATTGTAAGT	95 °C 3 min; 35 cycles of 94 °C 30 s, 54 °C 45 s, 72 °C 15 s; 72 °C 10 min; 10 °C soak	O’Donnell & Cigelnik (1997)
		T2	Reverse	TAGTGACCCTTGGCCCAGTTG
Calmodulin	CaM	CL1	Forward	GARTWCAAGGAGGCCTTCTC	95 °C 1 min; 35 cycles of 94 °C 30 s, 55 °C 30 s, 72 °C 15 s; 72 °C 10 min; 10 °C soak	O’Donnell et al. (2000b)
		CL2A	Reverse	TTTTTGCATCATGAGTTGGAC
Histone	H3	H3-la	Forward	ACTAAGCAGACCGCCCGCAGG	96 °C 2 min; 30 cycles of 92 °C 1 min, 60 °C 1 min, 72 °C 10 s; 72 °C 5 min; 10 °C soak	Roux et al. (2001)
		H3-lb	Reverse	GCGGGCGAGCTGGATGTCCTT
Internal transcribed spacer region of the rDNA	ITS	ITS1	Forward	CCGTAGGTGAACCTGCGG	95 °C 5 min; 35 cycles of 95 °C 30 s, 52 °C 30 s, 72 °C 10 s; 72 °C 5 min; 10 °C soak	White et al. (1990)
		ITS4	Reverse	TCCTCCGCTTATTGATATGC
RNA polymerase largest subunit	rpb1	F7	Forward	CRACACAGAAGAGTTTGAAGG	95 °C 5 min; 5 cycles of 95 °C 2 min, 58 °C 45 s, 72 °C 20 s; 5 cycles of 95 °C 2 min, 57 °C 45 s, 72 °C 20 s; 35 cycles of 95 °C 2 min, 56 °C 45 s, 72 °C 20 s; 72 °C 10 min; 10 °C soak	O’Donnell et al. (2010)
		G2R	Reverse	GTCATYTGDGTDGCDGGYTCDCC
RNA polymerase second largest subunit	rpb2	5f2	Forward	GGGGWGAYCAGAAGAAGGC	94 °C 90 s; 35 cycles of 94 °C 45 s, 57 °C 45 s, 72 °C 20 s; 72 °C 5 min; 10 °C soak	Reeb et al. (2004)
		7cf	Forward	ATGGGYAARCAAGCYATGGG		Liu et al. (1999)
		7cr	Reverse	CCCATRGCTTGYTTRCCCAT
		11ar	Reverse	GCRTGGATCTTRTCRTCSACC
translation elongation factor 1-alpha	tef1	EF-1	Forward	ATGGGTAAGGARGACAAGAC	94 °C 90 s; 35 cycles of 94 °C 45 s, 55 °C 45 s, 72 °C 15 s; 72 °C 10 min; 10 °C soak	O’Donnell et al. (1998b)
		EF-2	Reverse	GGARGTACCAGTSATCATG