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. 2023 Jun 7;14:1065609. doi: 10.3389/fmicb.2023.1065609

Figure 8.

Figure 8

Self-adjuvanted DEN VLPs elicit CD8 effector memory T cell responses. C57BL/6 mice were immunised subcutaneously with two doses of α-GalCer-DEN-3 VLP, DEN-3 VLP (delivered with Alum), α-GalCer alone or PBS. At day 21, spleens were harvested and analysed by FACS. (A) Representative plots showing the percentage of NKT cells (top) or CD8 T cells (middle) amongst viable (7-AAD-), CD19- lymphocytes. Bottom row depicts the percentage of CD8+ effector-memory (Tem) cells (CD44+ CD62L-) within the CD8 T cell population. (B) Graph depicts the percentage of CD8 Tem cells amongst CD8 T cells. (C) Represent the number of splenic CD8 Tem cells. Each point represents one animal with the bar showing the mean (B,C). Statistical significance between VLP (Alum) and VLP (α-GalCer) assessed via a two-tailed Mann Whitney test (*p < 0.05). (D) C57BL/6 mice were immunised either intramuscularly or subcutaneously with two doses of α-GalCer-DEN-3 VLP, DEN-3 VLP (delivered with Alum), α-GalCer alone or PBS. At day 42, spleens were harvested and analysed by FACS. (E) Graph depicts the percentage of CD8 TEM cells amongst CD8 T cells. (F) Statistical significance of injection route was assessed via way a two-way ANOVA with Bonferroni post-test correction (**p < 0.01).