FIG. 3.

Barrier enhancement by fingolimod via c-Abl tyrosine kinase. Left panel: HPAEC were transfected with 100 nM c-Abl or control siRNA and seeded onto Transwell inserts. After FTY720 stimulation (1 μM), FITC-dextran was added into the top chamber and incubated for 2 h. The fluorescent intensity of the bottom chamber was analyzed by fluorometry as per Methods. n = 4. *P < 0.031 versus control siRNA. Right panel: HPAEC were preincubated for 1 h with the c-Abl inhibitor, AG957 (20 μM), or vehicle control and then stimulated with FTY720 (1 μM) or vehicle. Data are representative of three independent experiments. Figure adapted from Wang and others (2011). AG957, c-Abl inhibitor; Con, control; FITC, fluorescein isothiocyanate; FTY720, fingolimod; HPAEC, human pulmonary artery endothelial cells; siRNA, small interfering RNA. Reproduced with permission of the © ERS 2022. European Respiratory Journal Jul 2011, 38 (1) 78-88; DOI: 10.1183/09031936.00047810. Copyright permission reference number is ERJPM076-2022-23.