Table 1.
Characteristics of Phage Isolated Against Carbapenem-Resistant Klebsiella pneumoniae ST258 Strains
| K751 | T765 | T751 | |
|---|---|---|---|
| Host strain | 14–751 | 14–765 | 14–751 |
| Maximum concentration (PFU/mL) | 1.0 × 1011 | 9.0 × 1011 | 2.0 × 1010 |
| Phenotypic profile | |||
| Temperature (°C) | −16 to 70 | −16 to 60 | −16 to 60 |
| pH | 3 to 11 | 3 to 11 | 4 to 11 |
| Adsorption constant, K (mL/min) | 2.1 × 10−10 | 5.1 × 10−10 | 1.1 × 10−10 |
| Adsorption at 10 min (%) | 28.4 | 24.4 | 12.1 |
| Burst size (PFU/bacterial) | 6 | 10 | 44 |
| Latent period (minutes) | 35 | 45 | 35 |
| Taxonomy | |||
| Family | Ackermannviridae | Ackermannviridae | Ackermannviridae |
| Genus | Taipeivirus | Taipeivirus | Taipeivirus |
| Genomic profile | |||
| Size (bp) | 157.100 | 157.384 | 157.384 |
| Depth | 125.1 × | 219.2 × | 274.7 × |
| Content GC (%) | 46.5 | 46.4 | 46.4 |
| Integrity (%) | 94.9 | 94.9 | 94.8 |
| ORF | 197 | 195 | 196 |
| CDS | 204 | 205 | 204 |
| Hypothetical proteins | 188 | 188 | 190 |
| Functional proteins | 23 | 24 | 21 |
| tRNA | 5 | 6 | 6 |
| sRNA | 1 | 1 | 1 |
| In silico replicative cycle (%) | |||
| PhageAI (lytic) | 93.45 | 93.05 | 93.05 |
| BachliB (lytic) | 97.02 | 97.02 | 97.02 |
CDS, coding DNA sequences; GC, guanine-cytosine content; ORFs, open reading frames; PFU, plaque-forming units; sRNA, small RNA; tRNA, transfer RNA.