Figure 5.

Comparison of toxicity of PCB52 and its human-relevant metabolites as assessed by MTT assay in (A) Primary astrocyte cultures from PND 1-3 Sprague-Dawley rats and (B) primary glial cells from PND 1-3 C57BL/6 mice. The inflection points observed at 1 and 5 μM of 4-OH-PCB52 and 4-OH-PCB52 sulfate respectively, in mouse primary glia are notable and are not as prominently seen in the rat primary astrocytes. Data were obtained from at least three biological replicates with each containing three technical replicates and are represented as mean percent cell viability ± SEM against concentration of PCB52, 4-OH-PCB52, or 4-OH-PCB52 sulfate.