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. 2023 Jun 4;13(10):3434–3450. doi: 10.7150/thno.81951

Figure 7.

Figure 7

HIIT preserves astrocyte p-AQP4 and n-AQP4 polarity. (A) Confocal immunofluorescence triple staining for RECA1 (red), AQP4 (white), and GFAP (green) (a). XZ-plane view of the 3D rendering images (b). p-AQP4 and depolarized AQP4 intensities were analyzed (c and d). The scale bar represents 10 μm. (B) Confocal immunofluorescence triple staining for NeuN (red), AQP4 (green), and GFAP (white) (a). 3D rendering images of the NueN/GFAP/AQP4. The green ball represents n-AQP4, and the blue surface represents depolarized AQP4 (b). n-AQP4 and depolarized AQP4 intensities were analyzed (c and d). A.U. indicates arbitrary units. The scale bar represents 5 μm. Data represent mean ± SEM, n = 12 slices from 6 animals. *P < 0.05 vs. Cont group, #P < 0.05 vs. STZ group.