Table 3.
Effects of cell-laden biomaterials on MSCs osteogenic differentiation induced by topography
| Cell type | Material | Surface patterns | Result | Ref |
|---|---|---|---|---|
| hMSCs | PI | Micro-patterns Width: 2-15μm Depth: 2μm |
15 μm ridges increased adipogenic differentiation whereas 2 μm ridges enhanced osteogenic differentiation. | 9 |
| Nano-patterns Diameter: 600μm Depth: 200nm Periodicity: 650nm |
Nano-patterns increased differentiation towards both osteogenic and adipogenic lineages. | 9 | ||
| PDTEC PS |
Co-continuous ribbons Spacing: 48±5μm Height: 200nm |
Co-continuous topographies favor cytoskeletal anisotropy, FA maturation and osteogenic differentiation. | 42 | |
| HA | Micro/nano hybrid structure Width: 28μm Space: 24μm Diameter:70-100nm |
The micro/nano hybrid structure significantly enhanced the cell behavior including the adhesion, proliferation and osteogenic gene expression. | 127 | |
| Quartz | Chiral geometry Linewidth: 2μm Spacing: 2μm Depth: 3μm |
Cell adhesion, proliferation, and differentiation are greatly enhanced for cells cultured on dextral geometry than those on sinistral geometry. | 131 | |
| Silicone | Periodic nanopillar arrays Diameter: 54-105nm Periodicity:70-201nm Height: 39-85nm |
The nanopillar arrays enhance osteogenic differentiation of hMSCs, dependent on the age of the donor. | 58 | |
| Multiscale hierarchical topography | The 0.5⊥3∥25 substrate, resembling collagen topography the most, exhibits the highest osteogenesis. | 132 | ||
| CDMs PDMS |
Wave-like structure Amplitude: 0.4, 2.2μm |
CDMs and topography synergistically enhances osteogenic differentiation. | 116 | |
| PDMS | Wave-like topographies Wavelength: 0.5,3,10,27μm |
Compared to W27, W3 showed the enhanced stiffness of stem cell, promoting higher degree of osteogenic differentiation. | 37 | |
| TiO2 nanotubes | TiO2 nanograin with the nanopore surface Width: 50-60nm Diameter: 30-40 nm |
The expression of p-ERK and p-CREB increased in the TiO2 nanograin with the nanopore surface compared to the micro rough and nanotube surfaces. | 119 | |
| Rat MSCs | HAp | Micropatterns Height: 11.38±0.58μm Length: 63.87±3.41μm Width: 43.31±2.55μm |
The micro-patterned topography and Sr-doping had a synergetic effect on the adhesion, growth and osteogenic differentiation of BMSCs. | 111 |
| BaTiO3/ poly-(l-lactic acid) fibrous scaffolds | Randomly oriented electrospun | The topographical structure and electrical activity have combining effects on cell attachment, growth, and osteogenic response. | 130 | |
| TiO2 nanorod array | Nanoscale geometry Length: 1.5μm Diameter: 100nm |
A TiO2 nanorod array promotes the osteogenic differentiation of MSCs, while a TiO2 ceramic with a smooth surface suppresses it. | 59 | |
| MSCs | PCL | Micro-grooves Width: 16μm Height: 6μm |
The space constraint inhibits the extension of actomyosin cytoskeleton, instead, pseudopodia lead to cell polarization. | 126 |
| Nano-grooves Width: 400nm Height: 500nm |
The adhesion induction leads to the formation of FAs, promoting the osteogenic differentiation of stem cells. | 126 |