Figure 6.

EV^Ldlr/EV^{Ldlr releaser} treatment ameliorates abnormal lipid metabolism in Ldlr^-/- mice: (A) Schematic illustration of the experimental procedure. Ldlr^-/- mice aged 6-8 weeks were first fed with HFD for 8 weeks, followed by the tail vein injection of PBS or indicated EVs weekly for 8 weeks as indicated. At the end of the experiments, mice were sacrificed for systemic analysis. (B) Representative images of serum appearance from mice treated as indicated. Analysis of (C) total cholesterol, (D) total triglyceride, (E) LDL-C, and (F) HDL-C in Ldlr^-/- mice treated as indicated. (G) Representative images of Oil Red O staining of the liver in Ldlr^-/- mice treated as indicated. Analysis of (H) plasma ALT and (I) AST. Data are presented as mean±SEM. n=6. *P<0.05 by one-way ANOVA. ns, no significant difference.