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. 2023 Jun 7;14:1148317. doi: 10.3389/fimmu.2023.1148317

Figure 1.

Figure 1

MYCN amplified tumors are abundantly infiltrated with immunosuppressive macrophages. (A, B) Representative images (A) and quantification of positive IHC staining (B) for CD68, CD20, CD3, CD4, and CD8 in tissue sections from early-stage MYCN-NA (n = 5), late-stage MYCN-NA (n= 7) and MYCN-A (n = 6) human NB; scale bar = 50μm. (C) The percentages of intratumoral CD11b+ myeloid cells, CD11b+Gr1- monocytes and CD11b+Gr1+ granulocytes, CD3+ T cells, CD4+ T cells, and CD8+ T cells in early-stage and late-stage murine NB9464 tumors (n = 4). (D, E) Relative mRNA expression of immunostimulatory (D) and immunosuppressive genes (E) in TAMs isolated from early-stage and late-stage NB9464 tumors (n =3). * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001, ns, not significant.