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. 2000 Apr 15;28(8):e28. doi: 10.1093/nar/28.8.e28

Figure 2.

Figure 2

Homogeneous end-point detection of PSA and mPSA amplification. Background subtracted terbium fluorescence of reactions containing no template (NTC), 10 000 molecules of PSA or 10 000 molecules of mPSA cDNA template before addition of POPO™-3. Black and gray columns denote reactions containing a PSA- or mPSA-specific probe, respectively. (B) Fluorescence intensity ratios after addition of POPO™-3. When PSA is used as template, the PSA-specific probe (black column) is cleaved and thus the addition of POPO™-3 does not affect the terbium signal at 545 nm. Accordingly, the sensitized POPO™-3 emission at 572 nm is less significant than in negative reactions. The result is the same when mPSA is used as template in an amplification reaction containing a mPSA-specific probe (gray column).