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. 2000 Apr 15;28(8):e28. doi: 10.1093/nar/28.8.e28

Figure 5.

Figure 5

Dual end-point assay. Aliquots of 1000 copies of PSA, mPSA or both double-stranded cDNAs were amplified in the presence of a PSA-specific TaqMan probe and a mPSA-specific terbium probe. Black and gray columns represent the background subtracted FAM and terbium signals, respectively, after 40 PCR cycles. There is no cross-talk between the two labels: a positive FAM signal (>260 counts) is detected only in the presence of a PSA target and a positive terbium signal (>46 500 counts) in the presence of a mPSA target. All reactions contained 250 ng of human genomic DNA.