Fig. 4.

Axonal mitochondrial size correlates with g-ratio in NAGM. A Mean percentage myelinated axons with mitochondria in post-mortem control grey matter (CGM) of control donors and normal appearing grey matter (NAGM) tissue of donors with progressive MS. B–D Analysis of cross-sectional axonal mitochondrial area (B), perimeter (C), and circularity (D). Icons indicate measured mitochondrial characteristics (red), violin plots depict the data distribution of all measurements, datapoints represent mean per donor (● = female (SPMS), ▲ = male (SPMS), △ = male (PPMS), color-coded, see Table 1), and boxplots show the median and inter quartile range (IQR). E Scatter plot showing the correlation between mitochondria radius and g-ratio. The size of the datapoints indicates the axon radius in µm. Number of control donors (CGM) is 4, number of donors with progressive MS (NAGM) is 6, and 6–40 mitochondria per donor (dependent on the number of mitochondria in the 100–200 axons that were measured in Fig. 3) were analysed. Statistics were performed using an unpaired Student’s t test (A), linear mixed model (B–D), or a Pearson’s r test (E)